الاثنين، 31 أغسطس 2015

Vaginal Wet Mount

Vaginal Wet Mount (webmed)

A vaginal wet mount (sometimes called a vaginal smear) is a test to find the cause of vaginitis, or inflammation of the vagina and the area around the vagina camera.gif (vulva). 

Vaginitis is often caused by an infection, but it may also be caused by a reaction to vaginal products such as soap, bath oils, spermicidal jelly, or douches. Vaginitis may cause symptoms such as vaginal itching, pain, or discharge.

Infections that can cause vaginitis are common and include:

  • Yeast infection. A vaginal yeast infection is caused by a type of yeast called Candida albicans. A yeast infection is also called a candida infection, or candidiasis. A vaginal yeast infection often causes itching and a white, lumpy discharge that looks like cottage cheese. It also causes pain with sexual intercourse. A yeast infection is not a sexually transmitted infection (STI).
  • Trichomoniasis. Trichomoniasis is an infection caused by a parasite (Trichomonas vaginalis). It is sometimes called trichomonas infection, trichomonal infection, or simply trich (say "trick"). Trichomoniasis causes a vaginal discharge that is yellow-green, foamy, and bad-smelling. Pain with sex or urination may be present. Lower belly pain may also be present. Trichomoniasis is spread by sexual contact and is an STI.
  • Bacterial vaginosis. Bacterial vaginosis is a change in the balance of bacteria that are normally present in the vagina. The vaginal discharge is thin and milky with a strong fishy odor. Many women have no symptoms. Bacterial vaginosis is not an STI.
  • Other sexually transmitted infections (STIs).Infections such as chlamydiagenital wartssyphilis,herpes simplex, and gonorrhea can also affect the vagina. These infections are found by doing other tests.

A vaginal sample may be tested by:

  • Wet mount. A sample of the vaginal discharge is placed on a glass slide and mixed with a salt solution. The slide is looked at under a microscope for bacteria, yeast cells, trichomoniasis (trichomonads), white blood cells that show an infection, or clue cells that show bacterial vaginosis.
  • KOH slide.KOH slide. A sample of the vaginal discharge is placed on a slide and mixed with a solution of potassium hydroxide (KOH). The KOH kills bacteria and cells from the vagina, leaving only yeast for a yeast infection.
  • Vaginal pH. The normal vaginal pH is 3.8 to 4.5. Bacterial vaginosis, trichomoniasis, and atrophic vaginitis often cause a vaginal pH higher than 4.5.
  • Whiff test. Several drops of a potassium hydroxide (KOH) solution are added to a sample of the vaginal discharge. A strong fishy odor from the mix means bacterial vaginosis is present. 
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Why It Is Done

A vaginal wet mount is done to find the cause of vaginal itching, burning, rash, odor, or discharge.

How To Prepare

Do not douche, use tampons, or use vaginal medicines for 24 hours before the test.

A vaginal wet mount is not done during your menstrual period. Menstrual blood on the slide can change the results.

If you are or might be pregnant, tell your doctor before the vaginal wet mount is done.

How It Is Done

You will take off your clothes below the waist and drape a gown around your waist. You will then lie on your back on an examination table with your feet raised and supported by stirrups. This allows your doctor to look at the genital area.

Your doctor will put a smooth, curved speculum into your vagina. The speculum gently spreads apart the vaginal walls, which allows your doctor to see the inside of the vagina and the cervix.

Samples of fluid inside the vagina are taken with a swab or spatula. The sample is put on a slide for testing.


How It Feels

You may feel some discomfort when the speculum is inserted, especially if your vagina is irritated and tender. There may be a small amount of bleeding after this test.

Risks

There are no problems from collecting a sample of vaginal secretions.

Results

A vaginal wet mount (sometimes called a vaginal smear) is a test to find the cause of vaginitis, or inflammation of the vagina and the area around the vagina (vulva). Your doctor may talk to you about the results after the test. If the sample needs to be looked at by a lab, the results may be ready in 1 to 2 days.


Vaginal discharge
Normal:

No abnormal vaginal discharge is present. A small amount of discharge is normal.

Abnormal:

A white, lumpy discharge that looks like cottage cheese may mean a vaginal yeast infection is present. A yellow-green, foamy discharge that has a bad odor may mean trichomoniasis is present. A thin, gray-white vaginal discharge with a strong fishy odor may mean bacterial vaginosis is present.

Wet mount
Normal:

No yeast, bacteria, trichomoniasis, orclue cells are found on the slide. White blood cells are not present or very low in number.

Abnormal:

High numbers of white blood cells often mean a vaginal infection. Yeast cells found on the wet mount mean a vaginal yeast infection is present. Trichomonads on the wet mount mean trichomoniasis is present. Clue cells means bacterial vaginosis is present. If many Gardnerella vaginalis bacteria are present, this also may mean bacterial vaginosis is present.

 

KOH slide
Normal:

No yeast is found.

Abnormal:

Yeast cells means a yeast infection is present.

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الخميس، 27 أغسطس 2015

Differences between Enzymes and Hormones

Differences between Enzymes and Hormones

Enzymes are the biological catalyst which speed up the rate of biochemical reactions without undergoing any changes.
Hormones are molecules, usually a peptide (eg: insulin) or steroid (eg: estrogen) that is produced in one part of an organisms and triggers a specific cellular reactions in target tissues and organs some distance away.
Differences between Enzymes and Hormones

S.N.

Enzymes

Hormones

1.Mostly enzymes perform reactions at the place of origin i.e. in cells where they are produced.Hormones perform activity at some distance away from the site of origin.
2.Enzymes are biological catalyst. They catalyze the biological reactions.Hormones are not catalyst. They simply initiate biochemical reactions.
3.All enzymes are generally proteins. There are some exceptions like ribozymes (RNA with catalytic activity).The hormones may be polypeptides, terpenoids, steroids, phenolics compounds or amines.
4.Enzymes are not translocate from one part to another part of cell.Most of the hormones show polar translocation.
5.As enzymes are catalyst, at the end of reaction they remain unchanged and can be reutilized.As hormones are not catalyst, they participate in biological reaction and their chemical composition is changed and cannot be reutilized as such.
6.They are macromolecules with higher molecular weight.They have only low molecular weight.
7.They are non-diffusible through cell membrane.They are diffusible through cell membrane.
8.They either act intracellularly or carried by some ducts to another site.Generally carried by blood to a target organ.
9.It increases the rate of metabolic physiological processes.They may be either excitatory or inhibitory in their action.
10.They catalyze reversible reactions.Hormone controlled reactions are not reversible.
11.Reaction rate increases with increase in their concentration up to a limit.Deficiency or overproduction of hormone causes metabolic disorders or diseases.
12.They act quickly.Some hormones are quick acting, while some are slow acting with a lag period.
13.They are not used in metabolic functions.They are used up in metabolic functions.
14.They cannot regulate morphogenesis.Generally regulate morphogenesis, especially secondary sex character. 
15.Examples:
– Oxidoreductases
– Transferases
– Hydrolases
Examples:
– Insulin,
– Glucagon,
– T3, T4,


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الثلاثاء، 25 أغسطس 2015

pin worms

Pinworms 





What are pinworms?

Pinworms are a type of parasite that lives in thedigestive system of humans. They are common throughout the world.

Adult pinworms are about 0.5 in. (12.7 mm) long and look like little white threads. Pinworm eggs are so tiny, you'd need a microscope to see them. 





What causes pinworm infection?

Most people get infected by accidentally swallowing pinworm eggs. Anyone can get pinworms, but they are most common in school-aged children. They are usually spread like this:

  • A child swallows pinworm eggs, and they travel to the child's intestines. In about a month, the eggs hatch into worms. At night the female worms crawl out the rectum and lay eggs around the child's anus.
  • When the worms lay eggs, it can cause itching. If the child scratches, the eggs can cling to the child's fingers and get stuck under the fingernails. 
  • The eggs then stick to things the child touches, such as clothing, dishes, toys, and furniture. The eggs can live 2 to 3 weeks outside the body.
  • When you touch something the child has touched, the eggs get on your hands. Then if you touch food or your mouth, you can swallow the eggs. This starts the cycle over again.

Pinworms spread easily in homes, day care centers, schools, and other places where groups of people spend time together. So if one person in your family has pinworms, others probably do too. 

It's possible to get pinworms by inhaling airborne eggs, but this is rare. It's also rare to get pinworms from a swimming pool.

Pinworms are spread from person to person. Pets don't get pinworms and can't spread them to humans.

What are the symptoms?

Many people with pinworms don't have symptoms and don't know that they're infected. When symptoms occur, the most common ones are:

  • Itching around the anus.
  • Restless sleep, because itching is often worse at night.

Pinworms can be annoying. But they don't carry disease, and they rarely cause serious health problems. Sometimes people get a skin infection from scratching.

How are pinworms diagnosed?

To find out if you have pinworms, your doctor will ask about your past health and check the skin around your anus. 

The doctor may ask you to do a transparent tape test at home. To do the test, you press a piece of clear, sticky tape on the skin around your anus in the morning before you get up. The doctor will put the tape under a microscope to look for pinworm eggs. You might need to repeat this test a few times.

How are they treated?

You can treat pinworms with over-the-counter or prescription medicine that kills the worms. Treatment can help keep you from getting infected again and from spreading the infection to other people.

You will probably need two doses, 2 weeks apart. That's because the medicine kills the worms but not the eggs. The second dose will kill any worms that hatch after the first treatment.

Pinworm medicine may not be safe for children younger than 2 and women who are pregnant or breast-feeding. So to reduce their risk of infection, a doctor may recommend that all other household members be treated with medicine.

Call your doctor if:

  • Medicine hasn't cleared up the infection.
  • The medicine is causing side effects.
  • You have new or worse symptoms. 

How can you keep from spreading pinworms or getting them again?

Pinworms spread easily and often come back. To reduce your chances of spreading the infection or getting infected again: 

  • Wash your hands carefully and often. Teach your children to do the same, especially after they use the toilet and before they handle food.
  • Keep your fingernails short, and don't scratch the itch. Wearing gloves at night may help prevent scratching. 
  • Bathe or shower every day.
  • Don't share or reuse towels or washcloths.
  • Change your underwear and bedding each morning. 
  • Wash clothes, bedding, and towels regularly. Dry them in a hot dryer.

If anyone in your household gets pinworms again, the whole family may need to take medicine.

الجمعة، 21 أغسطس 2015

lab notes guide to lab and diagnostic tests

اسم الكتاب : lab notes guide to lab and diagnostic tests





حجم الكتاب 1 ميجا بايت تقريبا اي انه سريع جدا في خلال 20 ثانية سيصبح معك كتاب رائع في التحاليل الطبية تتصفحه و تستخدمه و تستفيد بكل معلومة فيه بدون الحاجة لوجود انترنت على جهازك 
التحميل يدعم كل الاجهزة الموبايل و التابلت و اللاب توب و لا حاجة لي كلمات سر او اي شيء و لا حاجة ايضا لتسجيل بريدك الالكتروني بأي موقع فقط تضغط زر التحميل و يصبح الكتاب معك 






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